Bacteria Category by Gram Staining Dissertation

Bacteria Classification by Gram Staining

Bacteria Category By Gram Staining

THE AMERICAN UNIVERSITY OR COLLEGE IN CAIRO

BIOLOGY DIVISION

SCIENCE 453: BIOLOGY TO GET ENGINEERS STATEMENT No . you

Presented By simply: Karim A. Zaklama 92-1509 Sci. 453-01

24/2/96

Goal:

To test a sample of clinical prepared bacteria and categorise it according to Christian's gram great and gram negative classes and also by simply viewing it under a powerful microscope and oil immersions; classify the shape and note any special features.

Introduction:

Bacterias was classified into two groups in 1884 by Danish Bacteriologist Christian, gram positive and gram unfavorable by a staining technique the place that the ability to avoid de-coloration of Crystal Violet solution by alcohol could render the class of gram positive, and gram unfavorable if the bacteria is de-coloured. This could be mentioned by the last colour in the bacteria: a violet shade where Gram positive and a green colour from the Safranin added pending the de-colouring process.

Materials:

1 . Bacteria Sample 2 . Microscopic lense Slide 3. Gram Staining Kit and Wash Containers a. Very Violet Solution b. Iodine Solution c. 95% Ethyl Alcohol m. Safranin electronic. Distilled Water 4. Bibulous Blotting Conventional paper 5. Microscope 6. Petrol

Procedure:

A. Preparation:

1 . Bacteria can be cultivated upon agar jelly in an incubator at 25В°C for 24 hours. installment payments on your Obtain a microscope slide and with a toothpick, smear a skinny coat in the bacteria test onto the slide several. Cover the smear with a drop Very Violet and leave position for 20 seconds some. Wash from the stain with distilled normal water; drain and blot off the excess with bibulous daily news. 5. Apply Gram's Iodine on the smear and keep to symbolize 1 minute. 6. Drain the excess iodine and apply 95% Ethyl alcohol intended for 20 second duration or till the alcohol operates clearly in the slide. several. The smear should rinsed for a few just a few seconds with unadulterated water to halt the action of the alcoholic beverages. 8. Drain and mark off the excess with...